Other

Part:BBa_K277117

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.D2.13


3L.3_23.D2.13 is 858 bases long and is cloned into the pGem-T vector.

3L.3_23.D2.13 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D2.13 is a constituent of 3L.3_23.D2 (along with 3L.3_23.D2.01, 3L.3_23.D2.02, 3L.3_23.D2.03, 3L.3_23.D2.04, 3L.3_23.D2.05, 3L.3_23.D2.06, 3L.3_23.D2.07, 3L.3_23.D2.08, 3L.3_23.D2.09, 3L.3_23.D2.10, 3L.3_23.D2.11, 3L.3_23.D2.12, 3L.3_23.D2.14, 3L.3_23.D2.15, 3L.3_23.D2.16, and 3L.3_23.D2.17.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

forward_primer YCL009C_tagf1v1 (729..756)

mutation_affecting_coding_sequence YCL009C_re_remove_BlpI (744..752) removal of BlpI changed PCRTag

forward_primer YCL009C_tagf1v2 (729..756)

gene YCL009C (570..+1499) Regulatory subunit of acetolactate synthase%2C which catalyzes the first step of branched-chain amino acid biosynthesis%3B enhances activity of the Ilv2p catalytic subunit%2C localizes to mitochondria

loxP_site loxPsym_YCL009C (533..566)

gene YCL010C (-512..267) SaGa associated Factor 29kDa%3B Probable 29kKDa Subunit of SAGA histone acetyltransferase complex

Sequence (corresponds to coordinates 85079..85936 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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